By Richard B. Thompson (auth.), Chris D. Geddes, Joseph R. Lakowicz (eds.)
Topics in Fluorescence Spectroscopy, complex options in Fluorescence Sensing half B: Macromolecular Sensing, is the 10th quantity within the well known sequence Topics in Fluorescence Spectroscopy, edited by way of Drs. Chris D. Geddes and Joseph R. Lakowicz.
This quantity contains authoritative macromolecular analytical fluorescence sensing reports really good adequate to be beautiful to specialist researchers, but additionally attractive to the broader viewers of scientists in similar disciplines of fluorescence. This quantity at once compliments quantity nine within the sequence, Part A: Small Molecule Sensing.
Advanced options in Fluorescence Sensing half B: Macromolecular Sensing is a vital reference for any lab operating within the analytical fluorescence sensing box. All lecturers, bench scientists, and pros wishing to use the most recent and maximum within the consistently rising box of macromolecular fluorescence sensing will locate it a useful resource.
Topics in Fluorescence Spectroscopy, complex ideas in Fluorescence Sensing half B: Macromolecular Sensing subject matters include:
Protein-based Biosensors with Polarization established Transduction
Fluorescent Saccharide Sensors
Fluorescent Pebble Nano-Sensors
Aptamers for Macromolecular Sensing
About the Editors:
Chris D. Geddes is an affiliate Professor of Fluorescence Spectroscopy, Director of the Institute of Fluorescence, and affiliate Director of the heart for Fluorescence Spectroscopy on the college of Maryland Biotechnology Institute, clinical Biotechnology heart, Baltimore. he's the Editor-in-Chief of the Journal of Fluorescence and either the Editor-in-Chief and Founding Editor of the Who's Who in Fluorescence and Reviews in Fluorescence annual volumes, in addition to government Director of the Society of Fluorescence. Dr Geddes has released over a hundred clinical articles, papers, overview articles, books and ebook chapters at the rules and functions of fluorescence.
Joseph R. Lakowicz is Professor of Biochemistry on the college of Maryland tuition of medication, Baltimore, and Director of the guts for Fluorescence Spectroscopy. Dr. Lakowicz has released over four hundred clinical articles, has edited a variety of books, holds sixteen issued patents, and is the writer of the generally used textual content, ideas of Fluorescence Spectroscopy.
Read or Download Advanced Concepts in Fluorescence Sensing: Part B: Macromolecular Sensing PDF
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Extra info for Advanced Concepts in Fluorescence Sensing: Part B: Macromolecular Sensing
To date, GFP-based sensors have been extensively applied to investigate signal transduction. Sensors of kinase activity, second messengers and G proteins have been used to investigate many aspects of cell signaling. In addition, sensors have been developed to measure enzyme activities, membrane potential, redox-state, protease activity, and metabolite concentrations. However, GFP-based sensors for other molecules of intermediary metabolism, such as ATP, phosphocreatine, and phosphate, which are not yet available, would be extremely useful in understanding metabolic organization in cells.
S. (1990). Mapping of fluorescence anisotropy in living cells by ratio ilnaging: Application to cytoplasmic viscosity. Biophysical Journal, 57(2),231-240. , & Munoz de la Pena, A. (1985). Detenninations ofinorganic substances by lUlninescence methods. In S. G. ), Molecular Luminescence Spectroscopy, Part I: Methods and Applications (Vol. 77, pp. 371-546). New York: Wiley-Interscience. Fierke, C. , & Thompson, R. B. (2001). Fluorescence-based biosensing of zinc using carbonic anhydrase. BioMetals, 14, 205 - 222.
L. Remington, Sensitivity of the yellow variant of green fluorescent protein to halides and nitrate, Curro BioI. 9, R628-R629 (1999). 6. S. Jayaraman, P. M. Wachter, SJ. S. Verkman, Mechanism and cellular application of a green fluorescent protein-based halide sensor, J. BioI. Chem. 275, 6047-6050 (2000). 7. S. A. Y. Tsien, Circular permutation and receptor insertions within green fluorescent proteins, Proc. Nat!. Acad. Sci. USA 96,11241-11246 (1999). 8. J. Nakai, M. Ohkura, and K. Imoto, A high signal-to-noise Ca 2+ probe composed of a single green fluorescent protein, Nat.