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Download Cell Movement and Neoplasia. Proceedings of the Annual by M. De Brabander, M. Mareel and L. De Ridder (Eds.) PDF

By M. De Brabander, M. Mareel and L. De Ridder (Eds.)

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Read Online or Download Cell Movement and Neoplasia. Proceedings of the Annual Meeting of the Cell Tissue and Organ Culture Study Group, Held at the Janssen Research Foundation, Beerse, Belgium, May 1979 PDF

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Additional resources for Cell Movement and Neoplasia. Proceedings of the Annual Meeting of the Cell Tissue and Organ Culture Study Group, Held at the Janssen Research Foundation, Beerse, Belgium, May 1979

Example text

The balance between spontaneous a s sembly and centrosomal assembly is dependent on the cell type and experimental v a r i ables. Preponderance of centrosomal nucleation is associated with a rapid reconstruction of an organized cytoplasmic microtubule complex. In the mitotic cells, released from nocodazole block, microtubule nucleation is strictly limited to the vicinity of the kinetochores and centrosomes, although they Function of the Microtubule System 37 were completely free of microtubule remnants.

The cultures were carried in 75 c m plastic bottles. When the cells formed a confluent sheet and no mitoses could be observed, they were subcultivated into new bottles at a 1:2 split ratio. The nutrient medium was left unchanged between each subcultivation. Cell counts were performed after trypsinization with an electronic Coulter counter. 5 ml of culture medium and seeded into 30 mm plastic Petri dishes. Special care was taken to ensure equal and uniform cell density at the start of an experiment, in all culture dishes belonging to the same group.

1 3 ) . Pronounced centrosome to centrosome and centrosome to kinetochore bundles appear thus very quickly while the microtubules pointing to other directions do apparently not elongate. We interpret this preferential assembly of inter-MTOC bundles as reflecting a summation of the assembly promoting capacity of neighbouring M T 0 C s . T f f DISCUSSION We believe that these experiments provide the first sufficiently controlled data on the reassembly of the microtubule system in mammalian cultured cells following its complete destruction by a specific and reversible inhibitor of tubulin assembly.

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